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pME18ST-d5-HT1B
(Plasmid #53108)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME18ST

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5HT1B
  • Alt name
    Serotonin receptor 1B
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    5-HT1B (a.k.a. Dmel_CG15113, 5-HT-dro2B, 5-HT1BDro, 5-HT[1B]Dro, 5-HT[[1BDro]], 5-HT[[1B]], 5-HT[[1B]]DRO, 5-HT[[1B]]Dro, 5-HT[[dro2B]], 5HT-R2B, 5HT-dro2B, 5HT-dro[[2B]], 5HT1B, 5htdro2b, CG15113, CG30126, DRO2B, Dm5HTdro2B, Dmel\CG15113, Dmel_CG30126, d5-HT1B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT1B. It was cloned from transgenic fly that has UAS-d5-HT1B (Bloomington #27632) and can be considered a functional variant.

Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME18ST-d5-HT1B was a gift from Leslie Vosshall (Addgene plasmid # 53108 ; http://n2t.net/addgene:53108 ; RRID:Addgene_53108)

  • For your References section:

    Small molecule drug screening in Drosophila identifies the 5HT2A receptor as a feeding modulation target. Gasque G, Conway S, Huang J, Rao Y, Vosshall LB. Sci Rep. 2013;3:srep02120. doi: 10.1038/srep02120. 10.1038/srep02120 PubMed 23817146
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