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PA-DAD-wt
(Plasmid #53095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx4
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5238
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LOV(wt)-6aa-DAD
  • Alt name
    mVenus - 3x GS linker - LOV2-Jα (404-546) - 3x GS linker - DAD (1177-1222)
  • Alt name
    pTriEx-mVenus-LOV(wt)-6aa-DAD
  • Species
    M. musculus (mouse); Avena sativa
  • Mutation
    L47F and C49S in Venus
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • mVenus (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pTriExUP
  • 3′ sequencing primer pTriExDOWN
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses photoactivatable (PA) Diaphanous auto-regulatory domain (DAD) consisting of a fusion of mVenus, LOV2 Jα protein domain of Avena sativa Phototropin1, and Diaphanous auto-regulatory domain of mouse Diaphanous-related formin-1 (mDia1)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PA-DAD-wt was a gift from Klaus Hahn & Ronen Zaidel-Bar (Addgene plasmid # 53095 ; http://n2t.net/addgene:53095 ; RRID:Addgene_53095)

  • For your References section:

    An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Rao MV, Chu PH, Hahn KM, Zaidel-Bar R. Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24. 10.1002/cm.21115 PubMed 23677607
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