pJM656
(Plasmid #53086)

• Purpose: A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control
• Depositing Lab: Ding Xue
• Publication: Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. doi: 10.1016/j.cub.2012.05.052. Epub 2012 Jun 21.

Backbone

• Vector backbone: pPD 49.83
• Backbone size w/o insert (bp): 3648
• Total vector size (bp): 5724
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lactaherin
• Alt name: MFGe8
• Species: M. musculus (mouse)
• Mutation: deleted native signal sequence, deleted C-terminal domain,FG/AA WNL/AAA
• Entrez Gene: Mfge8 (a.k.a. MFG-E8, MP47, Mfgm, P47, SED1, lactadherin)
• Promoter: hsp-16.41
• Tags / Fusion Proteins:
  • GFP (w/ introns) (N terminal on insert)
  • signal sequence (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: hsp-16
• 3′ sequencing primer: unc-54

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutations in Lactadherin described in

C. Shao, V.A. Novakovic, J.F. Head, B.A. Seaton, G.E. Gilbert
Crystal structure of lactadherin C2 domain at 1.7A resolution with mutational and computational analyses of its membrane-binding motif
J. Biol. Chem., 283 (2008), pp. 7230–7241