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Addgene

pWUR703
(Plasmid #53082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-1b
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 3621
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TtAgo
  • Alt name
    TTHB0068
  • Species
    Thermus thermophilus
  • Insert Size (bp)
    2223
  • Mutation
    D478A D546A
  • GenBank ID
    AP008227
  • Promoter T7
  • Tag / Fusion Protein
    • Strep-II tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWUR703 was a gift from John van der Oost (Addgene plasmid # 53082 ; http://n2t.net/addgene:53082 ; RRID:Addgene_53082)

  • For your References section:

    DNA-guided DNA interference by a prokaryotic Argonaute. Swarts DC, Jore MM, Westra ER, Zhu Y, Janssen JH, Snijders AP, Wang Y, Patel DJ, Berenguer J, Brouns SJ, van der Oost J. Nature. 2014 Mar 13;507(7491):258-61. doi: 10.1038/nature12971. Epub 2014 Feb 16. 10.1038/nature12971 PubMed 24531762
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