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Addgene

pBullet-tgn-n
(Plasmid #53071)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53071 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBullet
  • Backbone manufacturer
    JBEI
  • Backbone size (bp) 8000
  • Vector type
    Synthetic Biology
  • Selectable markers
    Neomycin (select with G418) ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ACAAGTTTGTACAAAAAAGCAGGCTTC
  • 3′ sequencing primer ACCACTTTGTACAAGAAAGCTGGGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBullet-tgn-n was a gift from Joshua Heazlewood (Addgene plasmid # 53071 ; http://n2t.net/addgene:53071 ; RRID:Addgene_53071)

  • For your References section:

    The Plant Glycosyltransferase Clone Collection for Functional Genomics. Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD, Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller HV, Heazlewood1 JL. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577. 10.1111/tpj.12577 PubMed 24905498
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