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Addgene

pJIT163-2NLSCas9
(Plasmid #53064)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJIT163
  • Backbone manufacturer
    pGREEN
  • Backbone size w/o insert (bp) 3741
  • Vector type
    Plant expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4206
  • Mutation
    Rice codon optimized
  • Promoter 2x35S

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer 35S promoter
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJIT163-2NLSCas9 was a gift from Caixia Gao (Addgene plasmid # 53064 ; http://n2t.net/addgene:53064 ; RRID:Addgene_53064)

  • For your References section:

    Targeted genome modification of crop plants using a CRISPR-Cas system. Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. 10.1038/nbt.2650 PubMed 23929338
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