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Addgene

pU3-gRNA
(Plasmid #53063)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53063 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC-T
  • Backbone manufacturer
    CWBIO
  • Backbone size w/o insert (bp) 2700
  • Vector type
    CRISPR ; Plant expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OsU3 promoter + gRNA scaffold
  • Species
    rice U3 promoter
  • Insert Size (bp)
    493
  • Promoter rice U3

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU3-gRNA was a gift from Caixia Gao (Addgene plasmid # 53063 ; http://n2t.net/addgene:53063 ; RRID:Addgene_53063)
  • For your References section:

    Targeted genome modification of crop plants using a CRISPR-Cas system. Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. 10.1038/nbt.2650 PubMed 23929338