pZmU3-gRNA
(Plasmid
#53061)
-
PurposeTranscription of guide RNA (gRNA) in maize cells
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEasy-blunt
-
Backbone manufacturerTransgen
- Backbone size w/o insert (bp) 3929
-
Vector typeCRISPR ; Plant expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namezmU3 promoter - gRNA
-
Speciesmaize (corn)
-
Insert Size (bp)869
- Promoter maize U3
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pZmU3-gRNA was a gift from Caixia Gao (Addgene plasmid # 53061 ; http://n2t.net/addgene:53061 ; RRID:Addgene_53061) -
For your References section:
Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System. Liang Z, Zhang K, Chen K, Gao C. J Genet Genomics. 2014 Feb 20;41(2):63-8. doi: 10.1016/j.jgg.2013.12.001. Epub 2013 Dec 14. 10.1016/j.jgg.2013.12.001 PubMed 24576457
Map uploaded by the depositor.
