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pSP64-hERG1a D540K
(Plasmid #53057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3017
  • Total vector size (bp) 6497
  • Vector type
    Xenopus Oocyte Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Potassium voltage-gated channel subfamily H member 2
  • Alt name
    ERG1, HERG, LQT2, HERG1, Kv11.1, KCNH2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3480
  • Mutation
    changed Aspartate 540 to Lysine
  • Entrez Gene
    KCNH2 (a.k.a. ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64-hERG1a D540K was a gift from Michael Sanguinetti (Addgene plasmid # 53057 ; http://n2t.net/addgene:53057 ; RRID:Addgene_53057)

  • For your References section:

    Mutations of the S4-S5 linker alter activation properties of HERG potassium channels expressed in Xenopus oocytes. Sanguinetti MC, Xu QP. J Physiol. 1999 Feb 1;514 ( Pt 3):667-75. PubMed 9882738
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