pCEP4-hERG1a
(Plasmid #53052)

• Purpose: Expresses alpha subunit of hERG1a K channel in mammalian cells
• Depositing Lab: Michael Sanguinetti
• Publication: Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12329-33.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10185
• Total vector size (bp): 13665
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Potassium voltage-gated channel subfamily H member 2
• Alt name: ERG1, HERG, LQT2, HERG1, Kv11.1, KCNH2
• Species: H. sapiens (human)
• Insert Size (bp): 3480
• Entrez Gene: KCNH2 (a.k.a. ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-Fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the methionine of the insert sequence is located at bp 1092 in the full Addgene sequence. The annotations incorrectly notate a methionine in the CMV promoter as the beginning of the insert.