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PurposeExpresses beta subunit for IKs channel in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCEP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10185
- Total vector size (bp) 10575
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePotassium voltage-gated channel subfamily E member 1
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SpeciesH. sapiens (human)
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Insert Size (bp)390
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Entrez GeneKCNE1 (a.k.a. ISK, JLNS, JLNS2, LQT2/5, LQT5, MinK)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEP4-hKCNE1 was a gift from Michael Sanguinetti (Addgene plasmid # 53050 ; http://n2t.net/addgene:53050 ; RRID:Addgene_53050) -
For your References section:
Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks) potassium channel. Sanguinetti MC, Curran ME, Zou A, Shen J, Spector PS, Atkinson DL, Keating MT. Nature. 1996 Nov 7;384(6604):80-3. 10.1038/384080a0 PubMed 8900283
Map uploaded by the depositor.
