pHL1756
(Plasmid #53036)

• Purpose: PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR
• Depositing Lab: Han Lim
• Publication: Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: Lim lab
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GFP
• Species: E.coli
• Promoter: pConNoHindM12
• Tag / Fusion Protein:
  • glgC leader (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: GFP-R

Gene/Insert 2

• Gene/Insert name: tetR
• Promoter: pConNoHind
• Tag / Fusion Protein:
  • RBS(st3) (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: AspTerm

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PconNoHindM12 promoter is the same as PconNoHind except for two substitution mutations at the −10 and −35 sites that moderately decrease transcription.