pHL1747
(Plasmid
#53035)
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PurposePLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunknown
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Backbone manufacturerLim lab
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namemCherry
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SpeciesE.coli
- Promoter pLtetO-1
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Tag
/ Fusion Protein
- ydeHlead (N terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namecsrA
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SpeciesE. coli
- Promoter pLlacO-1
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Tag
/ Fusion Protein
- RBS(st7) (N terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL1747 was a gift from Han Lim (Addgene plasmid # 53035 ; http://n2t.net/addgene:53035 ; RRID:Addgene_53035)