pHL1529
(Plasmid #53027)

• Purpose: PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA
• Depositing Lab: Han Lim
• Publication: Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: Lim lab
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GFP
• Species: E.coli
• Promoter: pLlac0-1
• Tag / Fusion Protein:
  • glgC leader contains -61 to +8 relative to the start codon (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: GFP-R

Gene/Insert 2

• Gene/Insert name: tetR
• Promoter: pConNoHind
• Tag / Fusion Protein:
  • RBS(st3) (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: AspTerm

Gene/Insert 3

• Gene/Insert name: csrA
• Species: E. coli
• Promoter: pConNoHind
• Tag / Fusion Protein:
  • RBS(st2) (N terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CsrA binding sites and flanking sequences from glgC (-61 to +8 relative to the start codon) were fused to gfp, thereby enableing GlgC-GFP expression ("target expression") to be quantified by fluorescence.