pHL 1915
(Plasmid
#53022)
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PurposeAmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Backbone manufacturerLim lab
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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SpeciesE.coli
- Promoter pLlacO-1 and fimS fusion
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL 1915 was a gift from Han Lim (Addgene plasmid # 53022 ; http://n2t.net/addgene:53022 ; RRID:Addgene_53022)
Map uploaded by the depositor.