pHL 1278
(Plasmid #53018)

• Purpose: AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1
• Depositing Lab: Han Lim
• Publication: Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: Lim lab
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: Synthetic
• Promoter: pLtetO-1
• Tag / Fusion Protein:
  • RBS(st7) (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: mCherry-R

Gene/Insert 2

• Gene/Insert name: GFP
• Species: Aequorea victoria
• Promoter: pLlacO-1
• Tag / Fusion Protein:
  • RBS(st7) (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: AspTerm

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry