pHL 342
(Plasmid #53012)

• Purpose: PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp
• Depositing Lab: Han Lim
• Publication: Hussein et al Nucleic Acids Res. 2012 May 22.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: Lim lab
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: Synthetic
• Promoter: pLtetO-1

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: araC
• Species: E. coli
• Promoter: pLlacO-1

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Gene/Insert 3

• Gene/Insert name: ompC
• Alt name: target mRNA
• Species: E. coli
• Mutation: contains -81 to +36 relative to start codon
• Promoter: Plar
• Tag / Fusion Protein:
  • gfp (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: GFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry