pHL 1032
(Plasmid #52997)

• Purpose: pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
• Depositing Lab: Han Lim
• Publication: Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: Lim lab
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RhyB sRNA
• Species: E. coli
• Promoter: pLtetO-1

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: AspTerm ACTGCTCACAAGAAAAAAGGCACG

Gene/Insert 2

• Gene/Insert name: ompC
• Alt name: target mRNA (competitor)
• Species: E. coli
• Mutation: contains −81 to +36 bp relative to start codon
• Promoter: pCon
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: mCherry-R

Gene/Insert 3

• Gene/Insert name: sodB
• Alt name: target mRNA
• Species: E. coli
• Mutation: contains −56 to +141 bp relative to start codon
• Promoter: pLlacO-1
• Tag / Fusion Protein:
  • gfp (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: GFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry