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Addgene

pJM483
(Plasmid #52987)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52987 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD 49.83
  • Backbone size w/o insert (bp) 3648
  • Total vector size (bp) 5724
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lactaherin
  • Alt name
    MFGe8
  • Species
    M. musculus (mouse)
  • Mutation
    deleted native signal sequence, deleted C-terminal domain
  • Entrez Gene
    Mfge8 (a.k.a. MFG-E8, MP47, Mfgm, P47, SED1, lactadherin)
  • Promoter hsp-16.41
  • Tags / Fusion Proteins
    • GFP (w/ introns) (N terminal on insert)
    • signal sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer hsp-16
  • 3′ sequencing primer unc-54
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJM483 was a gift from Ding Xue (Addgene plasmid # 52987 ; http://n2t.net/addgene:52987 ; RRID:Addgene_52987)

  • For your References section:

    CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine expression on apoptotic and phagocytic cells. Mapes J, Chen YZ, Kim A, Mitani S, Kang BH, Xue D. Curr Biol. 2012 Jul 24;22(14):1267-75. doi: 10.1016/j.cub.2012.05.052. Epub 2012 Jun 21. 10.1016/j.cub.2012.05.052 PubMed 22727702
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