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ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
(Plasmid #52985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    phage
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 8523
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
  • Species
    Synthetic
  • Insert Size (bp)
    2252
  • Promoter ubc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer ubc (5'-CGC CGA TGA TTA TAT AAG GA-3')
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC was a gift from Robert Singer (Addgene plasmid # 52985 ; http://n2t.net/addgene:52985 ; RRID:Addgene_52985)

  • For your References section:

    Background free imaging of single mRNAs in live cells using split fluorescent proteins. Wu B, Chen J, Singer RH. Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615. 10.1038/srep03615 PubMed 24402470
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