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Addgene

Pcr4-12xMBS-PBS
(Plasmid #52984)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52984 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCR4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    12xMBS-PBS
  • Species
    Synthetic
  • Insert Size (bp)
    1369
  • Promoter none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13pUC-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Single MBS-PBS repeat sequence is:
GATCCTACGG TACTTATTG CCAAGAAAG CACGAGCAT CAGCCGTGC CTCCAGGTC GAATCTTCA AACGAGCAG ACGATATGG CGTCGCTCG CTCCAGTAT TCCAGGGTT CATCA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pcr4-12xMBS-PBS was a gift from Robert Singer (Addgene plasmid # 52984 ; http://n2t.net/addgene:52984 ; RRID:Addgene_52984)
  • For your References section:

    Background free imaging of single mRNAs in live cells using split fluorescent proteins. Wu B, Chen J, Singer RH. Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615. 10.1038/srep03615 PubMed 24402470