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Purposeprovide MBS-PBS alternating sequence
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5328
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name12xMBS-PBS
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SpeciesSynthetic
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Insert Size (bp)1369
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13pUC-rev (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Single MBS-PBS repeat sequence is:
GATCCTACGG TACTTATTG CCAAGAAAG CACGAGCAT CAGCCGTGC CTCCAGGTC GAATCTTCA AACGAGCAG ACGATATGG CGTCGCTCG CTCCAGTAT TCCAGGGTT CATCA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pcr4-12xMBS-PBS was a gift from Robert Singer (Addgene plasmid # 52984 ; http://n2t.net/addgene:52984 ; RRID:Addgene_52984) -
For your References section:
Background free imaging of single mRNAs in live cells using split fluorescent proteins. Wu B, Chen J, Singer RH. Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615. 10.1038/srep03615 PubMed 24402470
