pUAST-FOXO WT
(Plasmid
#52982)
-
Purposeinsect expression of FOXO WT
-
Depositing Labs
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepUAST
- Backbone size w/o insert (bp) 8904
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFOXO
-
SpeciesD. melanogaster (fly)
-
Entrez Genefoxo (a.k.a. Dmel_CG3143, 3143, Afx, Akh, CG3143, DFOXO, DfoxO, Dfoxo, DmQ95V5, Dmel\CG3143, FKHR, FOXO, FOXO1, FoxO, Foxo, Foxo1, Q95V55, anon-WO0118547.374, cg3143, dFOXO, dFoxO, dFoxo, dfoxO, dfoxo, fOXO, fd88A, foxO)
- Promoter Hsp70
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCasper-hs
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pUAST-FOXO WT was a gift from Marc Montminy & Biao Wang (Addgene plasmid # 52982 ; http://n2t.net/addgene:52982 ; RRID:Addgene_52982)