pUAST-HDAC4 3A
(Plasmid #52978)

• Purpose: insect expression of HDAC4 3A constitutive active mutant
• Depositing Labs: Marc Montminy, Biao Wang
• Publication: Wang et al Cell. 2011 May 13;145(4):596-606. doi: 10.1016/j.cell.2011.04.013.

Backbone

• Vector backbone: pUAST
• Backbone size w/o insert (bp): 8904
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HDAC4
• Species: D. melanogaster (fly)
• Mutation: S239,573,748A constitutively active*
• Entrez Gene: HDAC4 (a.k.a. Dmel_CG1770, CG1770, DHDAC4, Dmel\CG1770, GC1770, HDAC, HDAC4a, dHDAC4, dmHDA405, hdac4)
• Promoter: Hsp70
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCasper-hs
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HDAC4 from DGRC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note that there are some mismatches between the HDAC4 in this plasmid and the top BLAST hits for this gene in GenBank. The depositor maintains that the clone in this plasmid is what was acquired from the DGRC.