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Addgene

pUAST-SIK3 K70M
(Plasmid #52973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIK3
  • Alt name
    Salt-inducible kinase 3
  • Species
    D. melanogaster (fly)
  • Mutation
    K70M kinase dead mutant
  • Entrez Gene
    Sik3 (a.k.a. Dmel_CG42856, CG15072, CG18604, CG42856, Dmel\CG42856, Dmel_CG15072, Dmel_CG18604, EMK/KIAA0999, SIK, SIK3)
  • Promoter Hsp70
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-SIK3 K70M was a gift from Marc Montminy & Biao Wang (Addgene plasmid # 52973 ; http://n2t.net/addgene:52973 ; RRID:Addgene_52973)

  • For your References section:

    A hormone-dependent module regulating energy balance. Wang B, Moya N, Niessen S, Hoover H, Mihaylova MM, Shaw RJ, Yates JR 3rd, Fischer WH, Thomas JB, Montminy M. Cell. 2011 May 13;145(4):596-606. doi: 10.1016/j.cell.2011.04.013. 10.1016/j.cell.2011.04.013 PubMed 21565616
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