HL 1216
(Bacterial strain
#52938)
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PurposeMG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + ΔaraC_KanR
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Depositing Lab
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Publication
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 52938 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonenone
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + ΔaraC_KanR
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone
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SpeciesE.coli
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This strain is a precursor to HL 1249 (identical strain, except KanR cassette on chromosome allows for selection).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HL 1216 was a gift from Han Lim (Addgene plasmid # 52938) -
For your References section:
Direct comparison of small RNA and transcription factor signaling. Hussein R, Lim HN. Nucleic Acids Res. 2012 May 22. 10.1093/nar/gks439 PubMed 22618873