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Addgene

HL 3221
(Bacterial strain #52936)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 52936 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    none
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG 1655 + lacIq inserted into the chromosome at the intS site + ΔoxyS_KanR
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    none
  • Species
    E.coli

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This strain is a precursor to HL 3262 (identical strain, except KanR cassette on chromosome allows for selection).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HL 3221 was a gift from Han Lim (Addgene plasmid # 52936)

  • For your References section:

    Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298
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