HL 751
(Bacterial strain
#52932)
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PurposeMG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanR
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 52932 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backbonenone
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanR
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone
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SpeciesE.coli
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This strain is a precursor to HL 770 (identical strain, except KanR cassette on chromosome allows for selection).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HL 751 was a gift from Han Lim (Addgene plasmid # 52932) -
For your References section:
Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298