HL 713
(Bacterial strain
#52931)
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PurposeMG 1655 + lacIq_KanR inserted into the chromosome at the intS site
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 52931 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonenone
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)MG 1655 + lacIq_KanR inserted into the chromosome at the intS site
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone
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SpeciesE.coli
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This strain is a precursor to HL 716 (identical strain, except KanR cassette on chromosome allows for selection).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HL 713 was a gift from Han Lim (Addgene plasmid # 52931) -
For your References section:
Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298