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Purposeexpress P2X4 protein with pHluorin at 123rd position
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52926 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemP2X4-pHluorin123
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SpeciesM. musculus (mouse)
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Insert Size (bp)1953
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P2X4-pHluorin123 was a gift from Baljit Khakh (Addgene plasmid # 52926 ; http://n2t.net/addgene:52926 ; RRID:Addgene_52926) -
For your References section:
Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin. Xu J, Chai H, Ehinger K, Egan TM, Srinivasan R, Frick M, Khakh BS. J Gen Physiol. 2014 Jul;144(1):81-104. doi: 10.1085/jgp.201411169. Epub 2014 Jun 16. 10.1085/jgp.201411169 PubMed 24935743
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/52/52926/52926-map_SX2hlbRWoggm.pdf.940x940_q85_autocrop.png)