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Purposecontains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6147
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHAS6
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SpeciesH. sapiens (human)
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Insert Size (bp)747
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Entrez GeneRPS6 (a.k.a. S6, eS6)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-RPS6 (pDD1902) was a gift from Dirk Dittmer (Addgene plasmid # 52913 ; http://n2t.net/addgene:52913 ; RRID:Addgene_52913) -
For your References section:
Ribosomal protein S6 interacts with the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus. Chen W, Dittmer DP. J Virol. 2011 Sep;85(18):9495-505. doi: 10.1128/JVI.02620-10. Epub 2011 Jul 6. 10.1128/JVI.02620-10 PubMed 21734034
Map uploaded by the depositor.
