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Addgene

pGL3-hsp82
(Plasmid #52909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4786
  • Total vector size (bp) 5779
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila pseudoobscura hsp82 promoter
  • Species
    Drosophila pseudoobscura
  • Insert Size (bp)
    992
  • Promoter D pseudoobscura hsp82

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer ataggctgtccccagtgcaagt
  • 3′ sequencing primer tttcatagcttctgccaaccgaacgg
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-hsp82 was a gift from Zach Adelman (Addgene plasmid # 52909 ; http://n2t.net/addgene:52909 ; RRID:Addgene_52909)

  • For your References section:

    Germline excision of transgenes in Aedes aegypti by homing endonucleases. Aryan A, Anderson MA, Myles KM, Adelman ZN. Sci Rep. 2013;3:1603. doi: 10.1038/srep01603. 10.1038/srep01603 PubMed 23549343
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