pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
(Plasmid
#52903)
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PurposeBinary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLpC
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Backbone manufacturerAddgene
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameReg-L-IFP-F[2]+Venus
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SpeciesH. sapiens (human); Deinococcus radiodurans
- Promoter Unknown
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Tag
/ Fusion Protein
- L-IFP-F[2] (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLpC-Reg-L-IFP-F[2]+Venus (mammalian) was a gift from Stephen Michnick (Addgene plasmid # 52903 ; http://n2t.net/addgene:52903 ; RRID:Addgene_52903) -
For your References section:
An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Tchekanda E, Sivanesan D, Michnick SW. Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 2014 Apr 20. 10.1038/nmeth.2934 PubMed 24747815