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Addgene

p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
(Plasmid #52898)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p413GAL1
  • Backbone manufacturer
    Addgene
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ypd*1-L-IFP-F[2]
  • Species
    S. cerevisiae (budding yeast); Deinococcus radiodurans
  • Mutation
    Changed Tryptophane 80 to Alanine
  • Promoter GAL1
  • Tags / Fusion Proteins
    • L-IFP-F[2] (C terminal on insert)
    • L-IFP-F[2] (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p413GAL1-Ypd*1-L-IFP-F[2] (Yeast) was a gift from Stephen Michnick (Addgene plasmid # 52898 ; http://n2t.net/addgene:52898 ; RRID:Addgene_52898)

  • For your References section:

    An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Tchekanda E, Sivanesan D, Michnick SW. Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 2014 Apr 20. 10.1038/nmeth.2934 PubMed 24747815
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