pSLfa PUb TAL-KMO-L
(Plasmid #52887)

• Purpose: Left hand TALEN against Aedes aegypti KMO gene
• Depositing Lab: Zach Adelman
• Publication: Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371/journal.pone.0060082. Epub 2013 Mar 21.

Backbone

• Vector backbone: pSLfa
• Backbone size w/o insert (bp): 4916
• Total vector size (bp): 7747

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TALEN-KMO
• Species: Aedes aegypti
• Promoter: Ae aegypti polyubiquitin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: ATTACTCAAGCGTTTCCTCGT
• 3′ sequencing primer: CTCTACAAATGTGGTATGGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TALEN was designed and constructed by CELLECTIS BIORESEARCH (France) then cloned into our backbone using standard techniques

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry