pBABE lucIL6
(Plasmid #52884)

• Purpose: expresses a firefly luciferase mRNA fused to the 3' UTR of IL6
• Depositing Lab: Sheila Stewart
• Publication: Alspach et al Cancer Discov. 2014 Mar 26.

Backbone

• Vector backbone: pBABE hygro
• Backbone size w/o insert (bp): 5578
• Total vector size (bp): 7616
• Vector type: Mammalian Expression, Retroviral, Luciferase
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: firefly luciferase fused to IL6 3'UTR
• Species: H. sapiens (human); firefly
• Insert Size (bp): 1653
• Entrez Gene: IL6 (a.k.a. BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Nicholas Davidson, Washington University in St. Louis
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry