pAcRP67A prM/E YFV
(Plasmid
#52883)
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Purposeexpression of Yellow Fever Virus VLPs in baculovirus
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcGP67A
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Backbone manufacturerBD
- Backbone size w/o insert (bp) 9761
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameprM/E
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Speciesyellow fever virus
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Insert Size (bp)2000
- Promoter Polyhedrin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Polyhedrin forward
- 3′ sequencing primer polyhedrin reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence in this plasmid corresponds to the prM and E genes of yellow fever virus. The reference sequence for this plasmid is NC_002031.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcRP67A prM/E YFV was a gift from Yorgo Modis (Addgene plasmid # 52883 ; http://n2t.net/addgene:52883 ; RRID:Addgene_52883)