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pcDNA3.1(+) prME (JEV) sdm2
(Plasmid #52881)

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Item Catalog # Description Quantity Price (USD)
Plasmid 52881 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    prME
  • Species
    Japanese encephalitis virus
  • Insert Size (bp)
    2000
  • Mutation
    sdm2
  • Entrez Gene
    POLY (a.k.a. JEVgp1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JEV CH2195

The sequence in this plasmid corresponds to the prM and E genes of Japanese encephalitis virus strain CH2195LA. The reference sequence for this isolate of the virus is GenBank AF221499.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+) prME (JEV) sdm2 was a gift from Yorgo Modis (Addgene plasmid # 52881 ; http://n2t.net/addgene:52881 ; RRID:Addgene_52881)

  • For your References section:

    Viral membrane fusion and nucleocapsid delivery into the cytoplasm are distinct events in some flaviviruses. Nour AM, Li Y, Wolenski J, Modis Y. PLoS Pathog. 2013;9(9):e1003585. doi: 10.1371/journal.ppat.1003585. Epub 2013 Sep 5. 10.1371/journal.ppat.1003585 PubMed 24039574
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