pEF-Bos-FLAG RIG-I MIII
(Plasmid #52877)

• Purpose: Expresses RIG-I with a mutation to motif III in mammalian cells
• Depositing Lab: Curt Horvath
• Publication: Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 10.1074/jbc.M807365200. Epub 2009 Feb 11.

Backbone

• Vector backbone: pEF-Bos-Flag
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 8600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DDX58
• Alt name: RIG-I
• Alt name: retinoic acid-inducible gene 1
• Species: H. sapiens (human)
• Insert Size (bp): 4700
• Mutation: T409A, S411A
• GenBank ID: NM_014314
• Entrez Gene: RIGI (a.k.a. DDX58, RIG-I, RIG1, RLR-1, SGMRT2)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pEF-Bos-F
• 3′ sequencing primer: pEF-Bos-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry