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PurposeExpresses MDA5 with a mutation to motif III in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF-Bos-Flag
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 8600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIFIH1
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Alt nameMDA5
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Alt nameMelanoma Differentiation-Associated protein 5
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SpeciesH. sapiens (human)
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Insert Size (bp)3075
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MutationT488A S490A
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GenBank IDNM_022168
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Entrez GeneIFIH1 (a.k.a. AGS7, Hlcd, IDDM19, IMD95, MDA-5, MDA5, RLR-2, SGMRT1)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pEF-Bos-F
- 3′ sequencing primer pEF-Bos-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-Bos-FLAG MDA5 MIII was a gift from Curt Horvath (Addgene plasmid # 52876 ; http://n2t.net/addgene:52876 ; RRID:Addgene_52876) -
For your References section:
Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2. Bamming D, Horvath CM. J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 10.1074/jbc.M807365200. Epub 2009 Feb 11. 10.1074/jbc.M807365200 PubMed 19211564