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Addgene

pEF-Bos-FLAG MDA5 MI
(Plasmid #52875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-Bos-Flag
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 8600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IFIH1
  • Alt name
    MDA5
  • Alt name
    Melanoma Differentiation-Associated protein 5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3075
  • Mutation
    K335A, E658K
  • GenBank ID
    NM_022168
  • Entrez Gene
    IFIH1 (a.k.a. AGS7, Hlcd, IDDM19, IMD95, MDA-5, MDA5, RLR-2, SGMRT1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-Bos-FLAG MDA5 MI was a gift from Curt Horvath (Addgene plasmid # 52875 ; http://n2t.net/addgene:52875 ; RRID:Addgene_52875)

  • For your References section:

    Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2. Bamming D, Horvath CM. J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 10.1074/jbc.M807365200. Epub 2009 Feb 11. 10.1074/jbc.M807365200 PubMed 19211564
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