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Addgene

TIRAP-TAP_IRES_GFP
(Plasmid #52740)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV2.2
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Toll/IL-1 Receptor adaptor protein
  • Alt name
    TIRAP
  • Alt name
    Mal
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    AF378129.1 Mal
  • Entrez Gene
    TIRAP (a.k.a. BACTS1, Mal, MyD88-2, wyatt)
  • Tags / Fusion Proteins
    • 3x HA (C terminal on insert)
    • 3x TEV (C terminal on insert)
    • BirA target site (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BirA target site in this plasmid(or biotin acceptor peptide, BAP) is GLNDIFEAQKIEWHE

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TIRAP-TAP_IRES_GFP was a gift from Jonathan Kagan (Addgene plasmid # 52740 ; http://n2t.net/addgene:52740 ; RRID:Addgene_52740)

  • For your References section:

    A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. Bonham KS, Orzalli MH, Hayashi K, Wolf AI, Glanemann C, Weninger W, Iwasaki A, Knipe DM, Kagan JC. Cell. 2014 Feb 13;156(4):705-16. doi: 10.1016/j.cell.2014.01.019. 10.1016/j.cell.2014.01.019 PubMed 24529375
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