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PurposeL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMRBAD
- Backbone size w/o insert (bp) 4493
- Total vector size (bp) 4856
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameZ+CGFP
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Insert Size (bp)363
- Promoter PBAD
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer PBAD forward (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRBAD-Z-CGFP was a gift from Lynne Regan (Addgene plasmid # 52735 ; http://n2t.net/addgene:52735 ; RRID:Addgene_52735) -
For your References section:
Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. Magliery TJ, Wilson CG, Pan W, Mishler D, Ghosh I, Hamilton AD, Regan L. J Am Chem Soc. 2005 Jan 12;127(1):146-57. 10.1021/ja046699g PubMed 15631464
