pET11a-Z-NGFP
(Plasmid #52734)

• Purpose: IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay
• Depositing Lab: Lynne Regan
• Publication: Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57.

Backbone

• Vector backbone: pET11a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5641
• Total vector size (bp): 6256
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NGFP+Z-fusion
• Insert Size (bp): 615
• Promoter: T7
• Tag / Fusion Protein:
  • His tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry