pMXs-hs-3xHA-NHL only-LIN-41
(Plasmid
#52723)
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Purposeretroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tags
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLIN-41
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Alt nameTRIM71
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SpeciesH. sapiens (human)
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Insert Size (bp)960
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MutationNHL-only contains only an initiating methionine and amino acids 583–868
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Entrez GeneTRIM71 (a.k.a. LIN-41, LIN41)
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Tag
/ Fusion Protein
- 3xHA (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-hs-3xHA-NHL only-LIN-41 was a gift from Shinya Yamanaka (Addgene plasmid # 52723 ; http://n2t.net/addgene:52723 ; RRID:Addgene_52723) -
For your References section:
The let-7/LIN-41 pathway regulates reprogramming to human induced pluripotent stem cells by controlling expression of prodifferentiation genes. Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, Narita M, Srivastava D, Yamanaka S. Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 10.1016/j.stem.2013.11.001. Epub 2013 Nov 14. 10.1016/j.stem.2013.11.001 PubMed 24239284