degtag
(Bacterial strain #52705)

• Purpose: KIlac with lacZ C-terminal sssA degradation tag
• Depositing Lab: Tanja Kortemme
• Publication: Eames et al Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): n/a
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: degtag strain

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate the degtag strain, we introduced the ssrA tag to the C-terminus of LacZ, thus targeting the protein for degradation by the ClpXP and ClpAP proteases.