Oplac1 (37)-KI
(Bacterial strain
#52701)
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PurposeKIlac with first 37 bases from Oplac1
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 52701 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backbonena
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)n/a
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOplac1 (37)-KI strain
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of KILac with those of OpLac1.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Oplac1 (37)-KI was a gift from Tanja Kortemme (Addgene plasmid # 52701) -
For your References section:
Cost-benefit tradeoffs in engineered lac operons. Eames M, Kortemme T. Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083. 10.1126/science.1219083 PubMed 22605776