OpLac2-delta-6
(Bacterial strain
#52700)
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PurposeOpLac2 without the first 6 nucleotides of lacZ
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 52700 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonena
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)na
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOpLac2-Δ6 strain
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Oplac2Δ6 was erroneously synthesized missing the first 6 nucleotides of Oplac2. These deletions correspond to the first 2 N-terminal amino acid residues (Methionine and Threonine), and instead begin at the Methionine at position 3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OpLac2-delta-6 was a gift from Tanja Kortemme (Addgene plasmid # 52700) -
For your References section:
Cost-benefit tradeoffs in engineered lac operons. Eames M, Kortemme T. Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083. 10.1126/science.1219083 PubMed 22605776