OpLac2
(Bacterial strain
#52699)
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PurposeKIlac with a codon optimized lacZ using codons for a subset of amino acids read by transfer RNAs that are the most highly charged during amino acid starvation
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 52699 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonena
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)na
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOpLac2 strain
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Oplac2 was designed by a proprietary optimization strategy (developed by DNA 2.0) that incorporated codons for a subset of amino acids read by tRNAs that are the most highly charged during amino acid starvation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OpLac2 was a gift from Tanja Kortemme (Addgene plasmid # 52699) -
For your References section:
Cost-benefit tradeoffs in engineered lac operons. Eames M, Kortemme T. Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083. 10.1126/science.1219083 PubMed 22605776