KIlac
(Bacterial strain #52696)

• Purpose: MG1655 with Lac Operon removed from native locus and re-integrated at the attTn7 locus
• Depositing Lab: Tanja Kortemme
• Publication: Eames et al Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): n/a
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KIlac strain

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

KIlac refers to the knock-in of the wild-type operon into the attTn7 locus; this strain was used as the reference strain for all analyses. We constructed all knock-in strains using the approach of McKenzie and Craig. The knock-in contained the entire lac operon starting 75bp upstream of lacI and ending 100bp downstream of lacA. The ΔZYA strain was transformed with pGRG37. The plasmid carried both the lac operon and the transposon genes tnsABCD, which allow for a site-specific insertion at attTn7.