delta-ZYA
(Bacterial strain #52695)

• Purpose: MG1655 with Lac Operon removed from native locus
• Depositing Lab: Tanja Kortemme
• Publication: Eames et al Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): n/a
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ΔZYA strain

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MG1655 was transformed with pKD46 (carrying phage lambda Red recombinase) and a PCR fragment encoding a kanamycin resistance gene with flanking nucleotide sequences homologous to those flanking the lac operon. Upon recombination and selection for resistant strains, the kanamycin-resistant gene was eliminated following transformation with pCP20 (encoding the FLP recombinase), which is subsequently cured by growth at 30°C. The deletion in our ΔZYA strain started 20bp upstream of lacI and ran 40bp downstream of lacA.